New functional composite for the treatment of bacterial bone infection based on hydroxyapatite and titanium metal-organic framework
Abstract
"Hydroxyapatite (HA) is widely used in medicine, including orthopedics (e.g. bone defects treatment or controlled drug release) and dentistry (as a component of implants) due to its bioactivity, biocompatibility, and good osteoconductive properties. A nanohydroxyapatite coating applied on a metal implant beneficially affects the change in the surface properties of the implant-tissue interface. However, HA does not lead to the inhibition of bacterial infections, which are still one of the most common causes of failures in bone regeneration. As a solution to this problem, a new biocompatible composite with antibacterial properties, consisting of HA nanoparticles and titanium(IV)-based metal-organic framework with the acronym MIL-125(Ti)-NH2 has been proposed. The solvothermally obtained material was characterized using various techniques, including PXRD, IR, TEM, TG, and low-temperature N2 sorption. The biocompatibility of the composite was confirmed during in vitro tests on osteoblasts (U2-OS) and fibroblasts (L929) as model cell lines. The introduction of gentamicin, an antibiotic used in the treatment of bone bacterial infections, into the MIL-125(Ti)-NH2@HA system increased the antibacterial activity of the material against Staphylococcus aureus and Pseudomonas aeruginosa. Additionally, the nanoindentation study showed an increase in the plastic work value of MIL-125(Ti)-NH2@HA compared to pristine HA, suggesting that novel composite can be suc-cessfully used as a component in surface modifications of implants. "
Weronika Bodylska
Organising Committee Member
