The process of visualization of biological objects is directly related to the use of fluorescent probes. The efficiency of fluorescent probes is determined by the signal-to-noise ratio caused by autofluorescence and scattered light in the biological environment under constant external excitation in the ultraviolet or visible range. In addition, it is important to monitor the detection of post-treatment effects such as apoptosis, oncosis, etc. [1]. Another important point is to understand how nanoparticles (NPs) interact with cell membranes. Persistent emissions (PersL) NPs meet most of these requirements. One of the representative PersL NPs is ZnGa2O4:Cr3+ (ZGO:Cr) with emission in the red region, first reported by Bessière et al. [2]. In addition to the above-mentioned features, the process of interaction of NPs with cell membranes remains poorly understood. In the current work, ZGO:Cr synthesized by the hydrothermal method [3] with the addition of oleic acid to obtain a surface charge were investigated. The Zeta potential for obtained ZGO:Cr was tested before further investigation. The vibrational non-linear optics response of the lipid bilayer after adding PersL NPs into the solution by sum frequency generation spectroscopy were detected to determine changes in the interfacial systems