The expression profile of differentiation antagonizing non-proteincoding RNA (lncDANCR) in human bone marrowderived stem cells (BMSCS) – analysis during osteogenesis influenced by silicate phosphate hydroxyapatites co-doped with Li⁺, Eu³⁺, and Gd³⁺ ions
Abstract
Osteogenesis is a complex process orchestrated by multiple cellular and molecular actors, among which non-coding RNAs (ncRNAs) play crucial roles. They regulate various aspects of bone formation by modulating the expression of genes involved in cell differentiation, proliferation, and apoptosis [1], [2]. In our research, we focus on the importance of ncRNAs in the finely tuned regulation of osteogenesis driven by biomaterials. Recently, differentiation antagonizing nonprotein coding RNA (lncDANCR) has gained attention as a significant regulator of osteogenic differentiation. The molecule is known to inhibit the differentiation of mesenchymal stem cells (MSCs) into osteoblasts, highlighting its potential as a therapeutic target for enhancing bone regeneration and treating osteoporosis [3]. In the current study, we were interested in the influence of theranostic biomaterials, i.e., silicate phosphate hydroxyapatites co-doped with Li+, Eu3+, and Gd3+ ions on the lncDANCR expression profile in human bone marrow stem cells (BMSCs) during osteogenic differentiation. The cells used for the experiment had confirmed multipotency (SCC034, Merck/Sigma-Aldrich, Poland) and underwent osteogenesis. Transcript levels were determined using RT-qPCR technology. The lncRNA DANCR expression was compared with mRNA levels for RUNX-2, i.e., a transcriptional factor that serves as the master regulator of osteogenesis. Additionally, alongside the lncDANCR expression profile, several small non-coding RNAs were determined, including miR-7-5p, miR-17-5p, miR-21-5p, miR-30a-5p, miR-106a-5p, miR-124-3p, miR-125-5p, miR-320-3p, miR-410-3p, miR433-3p. The analyzed expression profile shows us complex molecular networks involved in bone formation and remodeling guided by theranostics’ biomaterials. Understanding how lncDANCR, RUNX-2 expression, and several miRNAs interact and regulate these processes can provide valuable insights into optimizing biomaterials for enhanced bone regeneration and therapeutic applications.
