Formation and properties of liposomes under the influence of MoS2 nanoparticles
Abstract
In is known that 2D-MoS2 nanoparticles can be used for imaging of cells. In our experiment, the interaction of 2D-MoS2 nanoparticles with cells was modelled using liposomes. Liposomes with different phospholipid composition are convenient models of the cell membrane, which are used to study the effects of drugs and other external factors. In our previous work [1], the influence of WS2 nanoparticles on the formation of liposomes and enhancement of optical signal was investigated. To obtained liposomes 18:1 (Δ9-Cis) PC (DOPC) 1,2-dioleoyl-sn-glycero-3-phosphocholine Avanti Polar Lipids was used. The procedure of dissolving the lipids in chloroform was used, followed by resuspension in water and sequential freezing with liquid nitrogen and thawing. The concentration of liposomes in the resulting suspension was 4 mMol. MoS2 nanoparticles were added in one case during the formation of liposomes (at the stage of dissolution in water), and in the second case - after the completion of the procedure. In this way, two types of samples were obtained - with nanoparticles inside the liposome and with nanoparticles on the surface of the liposome. Thus, 2D-MoS2 nanoparticles introduced into liposomes at the stage of their formation have luminescent properties in the visible range that are about an order of magnitude higher and 2-3 times smaller than liposomes with 2D-MoS2 nanoparticles localized on their surface. Only the MoS2 nanodots can produce luminescence in the visible range, so the particles that entered the liposome turned into nanodots. This is important for cell imaging in different model experiments with different types of particles. The spectral features of the obtained structures are discussed. Acknowledgement M. Olenchuk and G. Dovbeshko acknowledge NRFU project No 2021.01/0229 “Biophysical characteristics of circulating metastatic cells as potential targets of antimetastatic therapy”, O. Gnatyuk acknowledge Academy of Finland mobility project Decision number 358103 for financial support. References [1] Galyna Dovbeshko et al. Effect of 2D-WS2 Nanoparticles on a Local Electrical Field at a Membrane Vicinity: Vibrational Spectroscopy Data. The Journal of Physical Chemistry C Article ASAP. DOI: 10.1021/acs.jpcc.3c05693
