Prefabricated zirconia crowns are the response to the increasing esthetic demands put on modern pediatric dentistry. They are prefabricated crowns allowing full coronal coverage of both anterior and posterior deciduous teeth. Their convenience is particularly noticeable in the anterior section of the dental arch. Zirconia advantages are good aesthetics, high strength, biocompatibility, high wear, and corrosion resistance. They can be used in patients with Ni- Cr allergy or sensitivity. The disadvantage is high cost. The aim of the study is to analyze the composition, properties of the biomaterial of zirconia crown, its potential cytotoxic properties and ability to form a bacterial bio-film, which is crucial for maintaining oral health. The structure of zirconia crown was investigated based on X-Ray Powder Diffraction (XRPD) patterns. For microbiological assay a quantitative assessment of the adhesion ability of the strains analyzed and the formation of a mixed biofilm on the dental material used was conducted, followed by confocal microscopy. Lastly histology assay was performed using direct contact on the Balb/3T3 normal mouse fibroblast line. The material of zirconia crowns is characterized by a moderate impact on the surrounding tissues. Like stainless steel crowns used in pediatric dentistry, they al-low full coverage and show the lowest risk of clinical failure. The material itself demonstrates a low degree of biofilm adhesion.